Device

Part:BBa_K1919300:Design

Designed by: Shaobo Yang   Group: iGEM16_SCU-China   (2016-09-16)


livJ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 203
    Illegal AgeI site found at 485
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 366


Design Notes

update later


Source

LivJ was cloned from genome of E.coli DH5a. Please note that there is no such gene in genome of E.coli BL21

References